Characterization of the A component of Streptococcus zymogenes lysin.
نویسندگان
چکیده
By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis. The A component was found to be a protein possessing a molecular weight of 27,000, a sedimentation coefficient approximating 3.2S, and a net negative charge at physiological pH.
منابع مشابه
Characterization of the A Component of Streptococcus zymogenes Lysin PAUL
By utilizing conventional techniques of pressure ultrafiltration, gel filtration chromatography, diethylaminoethyl cellulose chromatography, and preparative polyacrylamide electrophoresis, the A component of the group D lysin produced by Streptococcus zymogenes has been purified to a state of apparent homogeneity when determined by the techniques of anionic and cationic disc gel electrophoresis...
متن کاملProduction of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes.
A synthetic medium has been developed in which Streptococcus faecalis var. zymogenes X-14 elaborates a lysin. The medium consists of 18 amino acids, lactose, six vitamins, adenine, guanine, uracil, and six salts. Glucose, and K(2)HPO(4) in excess of 0.5% (wt/vol), were inhibitory to lysin production; increasing concentrations of l-arginine-hydrochloride gave increasing yields of lytic activity.
متن کاملBacteriolysis and inhibition of gram-positive bacteria by components of Streptococcus zymogenes lysin.
The ability of the A and L components of Streptococcus zymogenes lysin to cause lysis or to inhibit growth of a variety of gram-positive bacteria has been examined. Frank lysis of some, but not all, strains of S. faecalis, S. faecium, and S. liquefaciens by L component alone was demonstrated. None of the strains of these species was lysed by A component alone. S. durans was not lysed by either ...
متن کاملEffect of teichoic acid on resistance to the membrane-lytic agent of Streptococcus zymogenes.
Davie, Joseph M. (Indiana University, Bloomington), and Thomas D. Brock. Effect of teichoic acid on resistance to the membrane-lytic agent of Streptococcus zymogenes. J. Bacteriol. 92:1623-1631. 1966.-The resistance of Streptococcus zymogenes to its own lytic agent has been shown to be due to the production of a specific, inhibitory teichoic acid. A survey of streptococcal strains showed that o...
متن کاملBacteriocin (hemolysin) of Streptococcus zymogenes.
The sensitivity of Streptococcus faecalis (ATTC 8043) to S. zymogenes X-14 bacteriocin depends greatly on its physiological age. Sensitivity decreases from the mid-log phase on and is completely lost in the stationary phase. The sensitivity of erythrocytes to the hemolytic capacity of the bacteriocin showed considerable species variation. The order of increasing sensitivity was goose < sheep < ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of bacteriology
دوره 107 2 شماره
صفحات -
تاریخ انتشار 1971